Journal: Stem Cell Research & Therapy

Article Title: Co-treatments of vitamin D and mesenchymal stem cells effectively alleviate the diabetic kidney disease through attenuating the SIRT1-mediated pathways

doi: 10.1186/s13287-025-04579-w

Figure Lengend Snippet: Effects of co-treatments with VD and MSCs on the renal injuries through enhancing the levels of VD metabolites in the STZ-induced T1DM mice model Note: A: Contents of serum VD metabolites as the 25(OH)D and 1,25(OH) 2 D; B: Contents of serum 8-OHdG and KIM1; C: Correlations between serum VD metabolites with insulin, FBG, 8-OHdG and KIM1; D: Contents of renal VD metabolites; E: Contents of renal 8-OHdG and KIM1; F: Correlations between renal VD metabolites with renal 8-OHdG and KIM1; G: mRNA expressions of related genes on the VD metabolism and renal injuries; H: Protein expressions of VDR, CYP24A1 and CYP27B1, and their relatively quantitative assessments of protein expressions on the grayscale values with the internal reference as GAPDH; I and K: Renal ZEB1 and ZEB2 by the immunohistochemical detection. VD: vitamin D, MSCs: mesenchymal stromal cells, STZ: streptozotocin, T1DM: type 1 diabetes, 8-OHdG: 8-hydroxy-2 deoxyguanosine, KIM1: kidney injury molecule 1, FBG: random blood glucose, ZEB1: zinc finger E-box binding homeobox 1, ZEB2: zinc finger E-box binding homeobox 2. All data was presented as mean ± standard deviation ( n = 8/group). One-way analysis of variance was performed to compare the differences among these four groups and followed by the Student-Newman-Keuls test between each two groups. *Compared to the Control group, P < 0.05; # Compared to the T1DM group, P < 0.05; & Compared to the MSCs-T1DM group, P < 0.05

Article Snippet: After blocking with 5% skim milk in the 10% TBST for 2 h at the room temperature, the PVDF membranes were incubated overnight at 4°C with the specific primary antibodies, namely PPAR-γ (no. 2435, Cell Signaling Technology, 1:1000), C/EBPα (no. 2295, Cell Signaling Technology, 1:1000), ALP (no. sc-365765, Santa Cruz, 1:100), Runx2 (no. 8486, Cell Signaling Technology, 1:1000), VDR (no. 12550, Cell Signaling Technology, 1:1000), CYP27B1 (no. sc-515903, Santa Cruz, 1:100), CYP24A1 (no. K002997P, Solarbio, 1:500), IL1β (no. GB11113, Servicebio, 1:1000), iNOS (no. GB115703 , Servicebio, 1:1000), COL1A1 (no. 72026, Cell Signaling Technology, 1:1000), α-SMA (no. GB111364 , Servicebio, 1:1000), TGFβ (no. 3711, Cell Signaling Technology, 1:1000), Fibronectin1 (no. GB114491 , Servicebio, 1:1000), Smad2/3 (no. 3102, Cell Signaling Technology, 1:1000), P-Smad2 (no. 18338, Cell Signaling Technology, 1:1000), P-Smad3 (no. 9520, Cell Signaling Technology, 1:1000), SIRT1 (no. 9475, Cell Signaling Technology, 1:1000), α-Tubulin (no. 11224-1-AP, Proteintech, 1:2000), and GAPDH (no. 5174, Cell Signaling Technology, 1:2000).

Techniques: Immunohistochemical staining, Binding Assay, Standard Deviation, Control

Journal: Cancer Communications

Article Title: Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1‐mediated MAPK signaling

doi: 10.1002/cac2.12187

Figure Lengend Snippet: SQLE accelerated the proliferation of CRC cells in vitro . A‐B. SQLE protein levels in HT29 (A) or RKO (B) cells after knockdown of SQLE (SQLE KD) were accessed by Western blotting analysis. shcontrol was served as control. C. Cell viabilities of HT29 cells after knockdown of SQLE (SQLE KD) were accessed by CCK8 assay. shcontrol was served as control. D‐E. Colony formation of HT29 cells after knockdown of SQLE (SQLE KD). shcontrol was served as control. F. Cell viabilities of RKO cells after knockdown of SQLE (SQLE KD) were accessed by CCK8 assay. shcontrol was served as control. G‐H. Colony formation of RKO cells after knockdown of SQLE (SQLE KD). shcontrol was served as control. I‐J. SQLE protein levels in HT29 (I) or RKO (J) cells after overexpression of SQLE (SQLE OE) were accessed by Western blotting analysis. Vector was served as control. K. Cell viabilities of HT29 cells after overexpression of SQLE (SQLE OE) were accessed by CCK8 assay. Vector was served as control. L‐M. Colony formation of HT29 cells after overexpression of SQLE (SQLE OE). Vector was served as control. N. Cell viabilities of RKO cells after overexpression of SQLE (SQLE OE) were accessed by CCK8 assay. Vector was served as control. O‐P. Colony formation of RKO cells after overexpression of SQLE (SQLE OE). Vector was served as control. Q‐R. Cell cycle analysis (G 0 /G 1 , S, G 2 /M phase) of HT29 cells after knockdown of SQLE (SQLE KD). shcontrol was served as control. S‐T. Cell cycle analysis (G 0 /G 1 , S, G 2 /M phase) of HT29 cells after overexpression of SQLE (SQLE OE). Vector was served as control. U. SQLE, NCEH1, LIPA, CYP24A1 and STAG2 protein level in shcontrol and SQLE KD HT29 cells by Western blotting analysis. β‐actin was used as loading control. Mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, no significance

Article Snippet: Membranes were overnight incubated with primary antibodies against SQLE (1:1000 dilution; Cat#12544‐1‐AP; Proteintech, Rosemont, IL, USA), NCEH1 (neutral cholesterol ester hydrolase 1; 1:500 dilution; Cat#A17741; Abclonal, Wuhan, Hubei, China), LIPA (lipase A; 1:500 dilution; Cat#12956‐1‐AP; Proteintech, Rosemont, IL, USA), CYP24A1 (cytochrome P450 family 24 subfamily A member 1; 1:500 dilution; Cat#21582‐1‐AP; Proteintech, Rosemont, IL, USA), ATP2B1 (ATPase plasma membrane Ca2+ transporting 1; 1:1000 dilution; Cat#A18688; Abclonal, Wuhan, Hubei, China), STAG2 (stromal antigen 2; 1:1000 dilution; Cat#19837‐1‐AP; Proteintech, Rosemont, IL, USA), CACNG4 (1:1000 dilution; Cat#A14118; Abclonal, Wuhan, Hubei, China), ERK1/2 (1:1000 dilution; Cat#9102; Cell Signaling Technolgy), P‐ERK1/2 (1:1000 dilution; Cat#9101; Cell Signaling Technolgy, Danvers, Massachusetts, USA), Tubulin (1:5000 dilution; Cat#E7; DSHB, lowa city, IA, USA) and β‐actin (1:5000 dilution; Cat#AC026; Abclonal, Wuhan, Hubei, China) after being blocked at 4°C.

Techniques: In Vitro, Knockdown, Western Blot, Control, CCK-8 Assay, Over Expression, Plasmid Preparation, Cell Cycle Assay

Journal: Cancer Communications

Article Title: Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1‐mediated MAPK signaling

doi: 10.1002/cac2.12187

Figure Lengend Snippet: Knockdown of SQLE reduced the levels of CYP24A1 and calcitriol. A. The volcano plots of RNA‐seq analysis between shcontrol and SQLE KD HT29 cells. 1516 genes were up‐regulated, while 1376 genes were down‐regulated. SQLE, CYP24A1, CACNG4, STAG2, ATP2B1, NCEH1, LIPA were marked. B. The schematic diagram of Vitamin D3 synthesis. Down‐regulated genes are marked in green; up‐regulated genes are marked in red. C. The relative mRNA expressions of SQLE, CYP24A1, LIPA and NCEH1 in shcontrol and SQLE KD HT29 cells. D. Intracellular cholesterol level of shcontrol and SQLE KD HT29 cells by cholesterol measurement. E. CYP24A1 expression in 8 paired CRC samples by real‐time PCR analysis. F. The correlation between SQLE and CYP24A1 in 8 paired CRC samples by linear regression analysis. G. The volcano plots of untargeted metabolomics analysis between shcontrol and SQLE KD HT29 cells, 22(S)‐Hydroxycholesterol, 7‐Ketocholesterol and calcitriol were marked. H‐I. Intracellular Ca 2+ concentration of shcontrol and SQLE KD HT29 cells. J‐K. Intracellular Ca 2+ concentration of shcontrol and SQLE KD HT29 cells with calcitriol treatment (0.5 μmol/L). DMSO was used as control. Fluo‐4 probe and Hoechst represented calcium signal and nucleus, respectively. Scale bars, 50 μm. Mean ± SEM. ***, P < 0.001; ns, no significance. KD, knockdown

Article Snippet: Membranes were overnight incubated with primary antibodies against SQLE (1:1000 dilution; Cat#12544‐1‐AP; Proteintech, Rosemont, IL, USA), NCEH1 (neutral cholesterol ester hydrolase 1; 1:500 dilution; Cat#A17741; Abclonal, Wuhan, Hubei, China), LIPA (lipase A; 1:500 dilution; Cat#12956‐1‐AP; Proteintech, Rosemont, IL, USA), CYP24A1 (cytochrome P450 family 24 subfamily A member 1; 1:500 dilution; Cat#21582‐1‐AP; Proteintech, Rosemont, IL, USA), ATP2B1 (ATPase plasma membrane Ca2+ transporting 1; 1:1000 dilution; Cat#A18688; Abclonal, Wuhan, Hubei, China), STAG2 (stromal antigen 2; 1:1000 dilution; Cat#19837‐1‐AP; Proteintech, Rosemont, IL, USA), CACNG4 (1:1000 dilution; Cat#A14118; Abclonal, Wuhan, Hubei, China), ERK1/2 (1:1000 dilution; Cat#9102; Cell Signaling Technolgy), P‐ERK1/2 (1:1000 dilution; Cat#9101; Cell Signaling Technolgy, Danvers, Massachusetts, USA), Tubulin (1:5000 dilution; Cat#E7; DSHB, lowa city, IA, USA) and β‐actin (1:5000 dilution; Cat#AC026; Abclonal, Wuhan, Hubei, China) after being blocked at 4°C.

Techniques: Knockdown, RNA Sequencing, Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Control

Journal: Cancer Communications

Article Title: Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1‐mediated MAPK signaling

doi: 10.1002/cac2.12187

Figure Lengend Snippet: Calcitriol and CYP24A1 restored the growth of SQLE KD HT29 cells through MAPK signaling. A. Cell viabilities of shcontrol and SQLE KD HT29 cells treated with calcitriol at 0.5, 1, 2.5, 5 μmol/L. DMSO was used as control. B. Cell viabilities of shcontrol and SQLE KD HT29 cells treated with calcitriol at 0.5, 1, 5, 10, 25, 50, 100, 250, 500 nmol/L for 96 h. DMSO was used as control. C. CYP24A1 was over‐expressed in shcontrol and SQLE KD HT29 cells. Cell viabilities of these cells were measured by CCK8. D‐E. CYP24A1 was over‐expressed in SQLE KD HT29 cells. Colony formation of these cells was measured. F. CYP24A1 was knocked down in shcontrol and SQLE KD HT29 cells. Cell viabilities of these cells were measured by CCK8. NC: negative control. G. GSEA set of MAPK signaling pathway by knockdown of SQLE in HT29 cells. H. The protein expression levels of SQLE, CACNG4, ERK1/2 and phosphorylation of ERK1/2 in shcontrol and SQLE KD HT29 cells were accessed by Western blotting. β‐actin was used as a loading control. I‐J. The protein expression levels of SQLE, CACNG4, CYP24A1, ERK1/2 and phosphorylation of ERK1/2 in shcontrol and SQLE KD HT29 xenograft tumors were accessed by Western blotting. Tubulin was used as a loading control. K. The protein expression levels of SQLE, CACNG4, CYP24A1, ERK1/2 and phosphorylation of ERK1/2 in SQLE KD HT29 cells treated with calcitriol (0.5 μmol/L) were accessed by Western blotting. DMSO was used as control. L‐M. The protein expression levels of SQLE, CACNG4, CYP24A1, ERK1/2 and phosphorylation of ERK1/2 in the cells used in C and F were accessed by Western blotting. N. Schematic diagram showing molecular mechanism of SQLE promoting CRC proliferation. SQLE increases the levels of calcitriol and CYP24A1.Then MAPK signaling is induced to accelerate CRC tumor growth. Mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001. KD, knockdown

Article Snippet: Membranes were overnight incubated with primary antibodies against SQLE (1:1000 dilution; Cat#12544‐1‐AP; Proteintech, Rosemont, IL, USA), NCEH1 (neutral cholesterol ester hydrolase 1; 1:500 dilution; Cat#A17741; Abclonal, Wuhan, Hubei, China), LIPA (lipase A; 1:500 dilution; Cat#12956‐1‐AP; Proteintech, Rosemont, IL, USA), CYP24A1 (cytochrome P450 family 24 subfamily A member 1; 1:500 dilution; Cat#21582‐1‐AP; Proteintech, Rosemont, IL, USA), ATP2B1 (ATPase plasma membrane Ca2+ transporting 1; 1:1000 dilution; Cat#A18688; Abclonal, Wuhan, Hubei, China), STAG2 (stromal antigen 2; 1:1000 dilution; Cat#19837‐1‐AP; Proteintech, Rosemont, IL, USA), CACNG4 (1:1000 dilution; Cat#A14118; Abclonal, Wuhan, Hubei, China), ERK1/2 (1:1000 dilution; Cat#9102; Cell Signaling Technolgy), P‐ERK1/2 (1:1000 dilution; Cat#9101; Cell Signaling Technolgy, Danvers, Massachusetts, USA), Tubulin (1:5000 dilution; Cat#E7; DSHB, lowa city, IA, USA) and β‐actin (1:5000 dilution; Cat#AC026; Abclonal, Wuhan, Hubei, China) after being blocked at 4°C.

Techniques: Control, Negative Control, Knockdown, Expressing, Phospho-proteomics, Western Blot

Journal: Cells

Article Title: Beneficial Effects of Vitamins K and D3 on Redox Balance of Human Osteoblasts Cultured with Hydroxyapatite-Based Biomaterials

doi: 10.3390/cells8040325

Figure Lengend Snippet: Reactive oxygen species (ROS) level in osteoblasts after incubation with hydroxyapatites and treated with vitamins D3, K1, MK4, and MK7 after 8, 12, 16, and 20 days. The results are expressed as the μM/min/mg of protein and are shown as the mean ± SD ( n = 5). The values for the control cells and the treated cells were significantly different according to unpaired Student’s t -test. * Statistically significant differences versus control, p < 0.05; ( a ) statistically significant differences versus group hydroxyapatites (Mx (Maxgraft), Cb (Cerabone), Ap (Apatos), Go (Gen-Os), respectively for graphs ( b – e )); p < 0.05.

Article Snippet: Vitamin K1 (Sigma–Aldrich, St. Louis, MO, USA), vitamin K2 (Menaquinone 4, MK4 (Sigma–Aldrich, St. Louis, MO, USA) and Menaquinone 7, MK7 (was chemically synthesized by the Pharmaceutical Research Institute, Warsaw, Poland)), and vitamin D3 (1,25-dihydroxyvitamin D3 (1,25(OH)2D3) (Santa Cruz Biotechnology, USA)), was added to cells.

Techniques: Incubation, Control

Journal: Cells

Article Title: Beneficial Effects of Vitamins K and D3 on Redox Balance of Human Osteoblasts Cultured with Hydroxyapatite-Based Biomaterials

doi: 10.3390/cells8040325

Figure Lengend Snippet: Glutathione (GSH) level in osteoblasts after incubation with hydroxyapatites and treated with vitamins D3, K1, MK4, and MK7 after 8, 12, 16, and 20 days. The results are expressed as the nmol/mg of protein and are shown as the mean ± SD ( n = 5). The values for the control cells and the treated cells were significantly different according to unpaired Student’s t -test. * Statistically significant differences versus control, p < 0.05; ( a ) statistically significant differences versus group hydroxyapatites (Mx (Maxgraft), Cb (Cerabone), Ap (Apatos), Go (Gen-Os), respectively for graphs ( b – e )); p < 0.05.

Article Snippet: Vitamin K1 (Sigma–Aldrich, St. Louis, MO, USA), vitamin K2 (Menaquinone 4, MK4 (Sigma–Aldrich, St. Louis, MO, USA) and Menaquinone 7, MK7 (was chemically synthesized by the Pharmaceutical Research Institute, Warsaw, Poland)), and vitamin D3 (1,25-dihydroxyvitamin D3 (1,25(OH)2D3) (Santa Cruz Biotechnology, USA)), was added to cells.

Techniques: Incubation, Control

Journal: Cells

Article Title: Beneficial Effects of Vitamins K and D3 on Redox Balance of Human Osteoblasts Cultured with Hydroxyapatite-Based Biomaterials

doi: 10.3390/cells8040325

Figure Lengend Snippet: GSH-Px activity in osteoblasts after incubation with hydroxyapatites and treated with vitamins D3, K1, MK4, and MK7 after 8, 12, 16, and 20 days. The results are expressed as the mU/mg of protein and are shown as the mean ± SD ( n = 5). The values for the control cells and the treated cells were significantly different according to unpaired Student’s t -test. * Statistically significant differences versus control, p < 0.05; ( a ) statistically significant differences versus group hydroxyapatites (Mx (Maxgraft), Cb (Cerabone), Ap (Apatos), Go (Gen-Os), respectively for graphs ( b – e )); p < 0.05.

Article Snippet: Vitamin K1 (Sigma–Aldrich, St. Louis, MO, USA), vitamin K2 (Menaquinone 4, MK4 (Sigma–Aldrich, St. Louis, MO, USA) and Menaquinone 7, MK7 (was chemically synthesized by the Pharmaceutical Research Institute, Warsaw, Poland)), and vitamin D3 (1,25-dihydroxyvitamin D3 (1,25(OH)2D3) (Santa Cruz Biotechnology, USA)), was added to cells.

Techniques: Activity Assay, Incubation, Control

Journal: Cells

Article Title: Beneficial Effects of Vitamins K and D3 on Redox Balance of Human Osteoblasts Cultured with Hydroxyapatite-Based Biomaterials

doi: 10.3390/cells8040325

Figure Lengend Snippet: 4-HNE level in osteoblasts after incubation with hydroxyapatites and treated with vitamins D3, K1, MK4, and MK7 after 8, 12, 16, and 20 days. The results are expressed as the nmol/mg of protein and are shown as the mean ± SD ( n = 5). The values for the control cells and the treated cells were significantly different according to unpaired Student’s t -test. * Statistically significant differences versus control. p < 0.05; ( a ) statistically significant differences versus group hydroxyapatites ((Mx (Maxgraft), Cb (Cerabone), Ap (Apatos), Go (Gen-Os), respectively for graphs ( b – e )), p < 0.05.

Article Snippet: Vitamin K1 (Sigma–Aldrich, St. Louis, MO, USA), vitamin K2 (Menaquinone 4, MK4 (Sigma–Aldrich, St. Louis, MO, USA) and Menaquinone 7, MK7 (was chemically synthesized by the Pharmaceutical Research Institute, Warsaw, Poland)), and vitamin D3 (1,25-dihydroxyvitamin D3 (1,25(OH)2D3) (Santa Cruz Biotechnology, USA)), was added to cells.

Techniques: Incubation, Control

Journal: Cells

Article Title: Beneficial Effects of Vitamins K and D3 on Redox Balance of Human Osteoblasts Cultured with Hydroxyapatite-Based Biomaterials

doi: 10.3390/cells8040325

Figure Lengend Snippet: DNA levels in osteoblasts after incubation with hydroxyapatites and treated with vitamins D3, K1, MK4, and MK7 after 4, 8, 12, 16, and 20 days. The results are expressed as the ng/mg of protein and are shown as the mean ± SD ( n = 5). The values for the control cells and the treated cells were significantly different according to unpaired Student’s t -test. * Statistically significant differences versus control, p < 0.05; ( a ) statistically significant differences versus hydroxyapatites (Mx (Maxgraft), Cb (Cerabone), Ap (Apatos), Go (Gen-Os), respectively for graphs ( b – e )), p < 0.05;

Article Snippet: Vitamin K1 (Sigma–Aldrich, St. Louis, MO, USA), vitamin K2 (Menaquinone 4, MK4 (Sigma–Aldrich, St. Louis, MO, USA) and Menaquinone 7, MK7 (was chemically synthesized by the Pharmaceutical Research Institute, Warsaw, Poland)), and vitamin D3 (1,25-dihydroxyvitamin D3 (1,25(OH)2D3) (Santa Cruz Biotechnology, USA)), was added to cells.

Techniques: Incubation, Control

Journal: Cells

Article Title: Beneficial Effects of Vitamins K and D3 on Redox Balance of Human Osteoblasts Cultured with Hydroxyapatite-Based Biomaterials

doi: 10.3390/cells8040325

Figure Lengend Snippet: ALP activity in osteoblasts after incubation with hydroxyapatites and treated with vitamins D3, K1, MK4, and MK7 after 4, 8, 12, 16, and 20 days. The results are expressed as the nmol/min/mg of protein and are shown as the mean ± SD ( n = 5). The values for the control cells and the treated cells were significantly different according to unpaired Student’s t -test. * Statistically significant differences versus control, p < 0.05; ( a ) statistically significant differences versus hydroxyapatites (Mx (Maxgraft), Cb (Cerabone), Ap (Apatos), Go (Gen-Os), respectively for graphs ( b – e )), p < 0.05.

Article Snippet: Vitamin K1 (Sigma–Aldrich, St. Louis, MO, USA), vitamin K2 (Menaquinone 4, MK4 (Sigma–Aldrich, St. Louis, MO, USA) and Menaquinone 7, MK7 (was chemically synthesized by the Pharmaceutical Research Institute, Warsaw, Poland)), and vitamin D3 (1,25-dihydroxyvitamin D3 (1,25(OH)2D3) (Santa Cruz Biotechnology, USA)), was added to cells.

Techniques: Activity Assay, Incubation, Control

Journal: Cells

Article Title: Beneficial Effects of Vitamins K and D3 on Redox Balance of Human Osteoblasts Cultured with Hydroxyapatite-Based Biomaterials

doi: 10.3390/cells8040325

Figure Lengend Snippet: OC levels in osteoblasts after incubation with hydroxyapatites and treated with vitamins D3, K1, MK4, and MK7 after 8, 12, 16, and 20 days. The results are expressed as the ng/mL of medium and are shown as the mean ± SD ( n = 5). The values for the control cells and the treated cells were significantly different according to unpaired Student’s t -test. * Statistically significant differences versus control, p < 0.05; ( a ) statistically significant differences versus group hydroxyapatites (Mx (Maxgraft), Cb (Cerabone), Ap (Apatos), Go (Gen-Os), respectively for graphs ( b – e )), p < 0.05. ( b ) statistically significant differences versus group vitamin D 3 with hydroxyapatites (Mx (Maxgraft), Cb (Cerabone), Ap (Apatos), Go (Gen-Os), respectively for graphs ( b – e )), p < 0.05.

Article Snippet: Vitamin K1 (Sigma–Aldrich, St. Louis, MO, USA), vitamin K2 (Menaquinone 4, MK4 (Sigma–Aldrich, St. Louis, MO, USA) and Menaquinone 7, MK7 (was chemically synthesized by the Pharmaceutical Research Institute, Warsaw, Poland)), and vitamin D3 (1,25-dihydroxyvitamin D3 (1,25(OH)2D3) (Santa Cruz Biotechnology, USA)), was added to cells.

Techniques: Incubation, Control

Journal: Scientific Reports

Article Title: Quantitative NMR (qNMR) spectroscopy based investigation of the absolute content, stability and isomerization of 25-hydroxyvitamin D2/D3 and 24( R ),25-dihydroxyvitamin D2 in solution phase

doi: 10.1038/s41598-022-06948-4

Figure Lengend Snippet: Calculation of qNMR absolute content of analytes 1 , 2 and 4 .

Article Snippet: 25-Hydroxyvitamin D2 (1, Cat No. HY-3249, Batch No. 16275), 25-Hydroxyvitamin D3 (4, Cat No.HY-32351A/CS-0847, Batch No. 43822) were purchased from MedChem Express.

Techniques:

Journal: Scientific Reports

Article Title: Quantitative NMR (qNMR) spectroscopy based investigation of the absolute content, stability and isomerization of 25-hydroxyvitamin D2/D3 and 24( R ),25-dihydroxyvitamin D2 in solution phase

doi: 10.1038/s41598-022-06948-4

Figure Lengend Snippet: Structures of Vitamin D vitamers ((25-hydroxyvitamin D2 (1); 24 R ,25-dihydroxyvitamin D2 (2); 25-dihydroxyvitamin D3 (4)) and the corresponding isomerization pre-vitamin products.

Article Snippet: 25-Hydroxyvitamin D2 (1, Cat No. HY-3249, Batch No. 16275), 25-Hydroxyvitamin D3 (4, Cat No.HY-32351A/CS-0847, Batch No. 43822) were purchased from MedChem Express.

Techniques:

Journal: Scientific Reports

Article Title: Quantitative NMR (qNMR) spectroscopy based investigation of the absolute content, stability and isomerization of 25-hydroxyvitamin D2/D3 and 24( R ),25-dihydroxyvitamin D2 in solution phase

doi: 10.1038/s41598-022-06948-4

Figure Lengend Snippet: Overlay of 8 measurements at intervals of 8 h, for 25-hydroxyvitamin D2 (1).

Article Snippet: 25-Hydroxyvitamin D2 (1, Cat No. HY-3249, Batch No. 16275), 25-Hydroxyvitamin D3 (4, Cat No.HY-32351A/CS-0847, Batch No. 43822) were purchased from MedChem Express.

Techniques:

Journal: Scientific Reports

Article Title: Quantitative NMR (qNMR) spectroscopy based investigation of the absolute content, stability and isomerization of 25-hydroxyvitamin D2/D3 and 24( R ),25-dihydroxyvitamin D2 in solution phase

doi: 10.1038/s41598-022-06948-4

Figure Lengend Snippet: New signals after 56 h in the CDCl 3 solution of molecule 25-hydroxyvitamin D3 (4).

Article Snippet: 25-Hydroxyvitamin D2 (1, Cat No. HY-3249, Batch No. 16275), 25-Hydroxyvitamin D3 (4, Cat No.HY-32351A/CS-0847, Batch No. 43822) were purchased from MedChem Express.

Techniques:

Journal: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

Article Title: Comprehensive and quantitative profiling of B vitamins and related compounds in the mammalian liver

doi: 10.1016/j.jchromb.2019.121884

Figure Lengend Snippet: Mass spectrometer parameters for identification of B vitamins and 1C-related amines.

Article Snippet: Deuterated internal standards (IS); pyridoxine-d4 (PND) and thiamine-d3 pyrophosphate chloride (TPPD), were purchased from Toronto Research Chemicals Inc. (Toronto, Canada).

Techniques: Mass Spectrometry